Gene transcriptions/Elements/MYB recognitions

A rosette of Arabidopsis thaliana with the inflourescence just emerging at the centre is imaged. Credit: Quentin Groom.{{free media}}

"Another class of cis-acting elements is represented by Boxes P and L from the parsley phenylalanine ammonia-lyase (PAL) and 4-coumarate:CoA ligase (4CL) genes and the H-box from the bean chalcone synthase gene Chs15 [23,24]."

"The combination of an [ACGT-containing element] ACE and a MRE confers light responsiveness to the CFI, F3H and FLS promoters."

Consensus sequences

"These elements fit the type II MYB consensus sequence A(A/C)C(A/T)A(A/C)C, suggesting that they are MYB recognition elements (MREs)."

The consensus sequence for the RRE is 5'-CATCTG-3'.

The consensus sequence for the ACE is 5'-CACGT-3'.

Flavonols

"Chalcone synthase (CHS), chalcone flavanone isomerase (CFI), flavanone 3-hydroxylase (F3H) and flavonol synthase (FLS) catalyze successive steps in the biosynthetic pathway leading to the production of flavonols."

In "Arabidopsis thaliana all four corresponding genes are coordinately expressed in response to light, and are spatially coexpressed in siliques, flowers and leaves."

"Light regulatory units (LRUs) sufficient for light responsiveness were identified in all four promoters."

A "R response element (RRE) was identified in the CHS promoter."

"Only the ACE from the CFI promoter shows flanking sequences giving rise to a symmetrical G-box (Foster et al., 1994; Menkens et al., 1995) that also fits the consensus of the R motif (CANNTG; Blackwell and Weintraub, 1990; Bodeau and Walbot, 1996) which is recognized by [basic helix-loop-helix] BHLH factors."

The gain-of-function for the FLS gene (MRE, ACE) lies between -97 and -42.

For the F3H gene the ACE lies between -463 and -382 and the MRE lies between -99 and -63.

For the CFI gene the ACE, MRE lie between -99 and -39.

For the CHS gene the ACE, RRE, MRE lie between -106 and -69.

See also

• Gene transcriptions/Boxes/Hs