Gene transcriptions/Elements/MYB recognitions
"Another class of cis-acting elements is represented by Boxes P and L from the parsley phenylalanine ammonia-lyase (PAL) and 4-coumarate:CoA ligase (4CL) genes and the H-box from the bean chalcone synthase gene Chs15 [23,24]."
"The combination of an [ACGT-containing element] ACE and a MRE confers light responsiveness to the CFI, F3H and FLS promoters."
Consensus sequences[edit | edit source]
"These elements fit the type II MYB consensus sequence A(A/C)C(A/T)A(A/C)C, suggesting that they are MYB recognition elements (MREs)."
The consensus sequence for the RRE is 5'-CATCTG-3'.
The consensus sequence for the ACE is 5'-CACGT-3'.
Flavonols[edit | edit source]
"Chalcone synthase (CHS), chalcone flavanone isomerase (CFI), flavanone 3-hydroxylase (F3H) and flavonol synthase (FLS) catalyze successive steps in the biosynthetic pathway leading to the production of flavonols."
In "Arabidopsis thaliana all four corresponding genes are coordinately expressed in response to light, and are spatially coexpressed in siliques, flowers and leaves."
"Light regulatory units (LRUs) sufficient for light responsiveness were identified in all four promoters."
A "R response element (RRE) was identified in the CHS promoter."
"Only the ACE from the CFI promoter shows flanking sequences giving rise to a symmetrical G-box (Foster et al., 1994; Menkens et al., 1995) that also fits the consensus of the R motif (CANNTG; Blackwell and Weintraub, 1990; Bodeau and Walbot, 1996) which is recognized by [basic helix-loop-helix] BHLH factors."
The gain-of-function for the FLS gene (MRE, ACE) lies between -97 and -42.
For the F3H gene the ACE lies between -463 and -382 and the MRE lies between -99 and -63.
For the CFI gene the ACE, MRE lie between -99 and -39.
For the CHS gene the ACE, RRE, MRE lie between -106 and -69.
See also[edit | edit source]
References[edit | edit source]
- Paul J Rushton and Imre E Somssich (August 1998). "Transcriptional control of plant genes responsive to pathogens". Current Opinion in Plant Biology 1 (4): 311-5. doi:10.1016/1369-5266(88)80052-9. http://arquivo.ufv.br/dbv/pgfvg/bve684/htms/pdfs_revisao/estresse/transcriptional.pdf. Retrieved 5 November 2018.
- Ulrike Hartmann, Martin Sagasser, Frank Mehrtens, Ralf Stracke and Bernd Weisshaar (January 2005). "Differential combinatorial interactions of cis-acting elements recognized by R2R3-MYB, BZIP, and BHLH factors control light-responsive and tissue-specific activation of phenylpropanoid biosynthesis genes". Plant Molecular Biology 57 (2): 155–171. doi:10.1007/s11103-004-6910-0. http://pubman.mpdl.mpg.de/pubman/item/escidoc:1222162/component/escidoc:1222161/hartmann_weisshaar_pmb_2005.pdf. Retrieved 10 November 2018.