Talk:WikiJournal of Medicine/Cell disassembly during apoptosis

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<meta name='citation_doi' value='10.15347/wjm/2017.008'>

Article information

Authors: Aaron Smith[i][a], Michael AF Parkes[i][a], Georgia K Atkin-Smith[a]  , Rochelle Tixeira[a]  , Ivan KH Poon[a][ii]  

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  1. ↑ 1.0 1.1 1.2 1.3 1.4 Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, Victoria, Australia.
  1. ↑ 1.0 1.1 contributed equally to the work
  2. ↑ i.poonAt signlatrobe.edu.au

 

Plagiarism check

checkY Done The initial plagiarism check revealed some similarity to previous publications by the authors: see the PDF of the plagiarism check report here.

Subsequently, an edit was made which resolved any resemblances. --Steven Fruitsmaak (Reply) 21:53, 24 June 2017 (UTC)[reply]

Editor's comments


Comments by Michaël R. Laurent, MD PhD ,


I would first like to thank the authors for their excellent submission on Cell disassembly during cell death. I think the illustration provided in this submission could be used to enhance several Wikipedia articles including Apoptosis and Bleb (cell biology) (I've added these Wikipedia articles into your submission’s infobox). Some additional articles or redirects can also be created which were not yet in Wikipedia including the term Apoptopodia. I think it is a considerable advancement beyond previous available schemes such as this one: File:Apoptosis.png.

I have the following initial comments:

1. The article seems to be about Cell disassembly during apoptosis mainly; the image and accompanying text do not seem to apply to cell necrosis or any other forms of cell death e.g. necroptosis? In that case I would suggest changing the Title to: Cell disassembly during apoptosis instead of “cell death”.

Response

I agree with the suggested change of title.

2. The article requires a short Abstract. I refer you for a recent example to the article: Eukaryotic and prokaryotic gene structure.

Response

The disassembly of a dying cell into smaller fragments is a fundamental biological process during apoptosis. Recently, a number of distinct morphologic changes have been identified that could mediate the fragmentation of an apoptotic cell. Presented here is a figure that describe the progression of apoptotic cell disassembly.

3. In Step 1: apoptotic membrane blebbing, two types are described i.e. small and large dynamic membrane blebs. It is mentioned in the text that larger blebs develop in later stages. Can these two types of blebs coexist and if so, should the image you submitted by altered accordingly? If the larger blebs occur during later stages, would it then be useful in the Image to put the two types of blebs next to each other instead of having them arranged one above the other?

Response

For some cell types, such as T cells, they could first display small membrane blebs, and then large dynamic membrane blebs. It is possible they could coexist momentarily but usually one after the other. However, cells like monocytes and melanocytes only form small membrane blebs prior to Step 2. We try to simplify the process to show there are two types of membrane blebs and avoid the complexity as described above. It is not incorrect to put them next to each other instead and we can change that if necessary.

4. Could you add a short explanation in the text regarding efferocytosis? I think it would be useful for our readership to learn about this specific term for phagocytosis of apoptotic cells.

Response

Is it possible to add that at the end of paragraph 1 “…and promote efficient clearance of apoptotic cells (a process known as efferocytosis).”

5. Finally I've made a few minor spelling and grammar changes and adjusted the references and lay-out of your article according to our Journal’s style. The rest of the text nicely summarizes the apoptotic cell disassembly process, thank you again for this nice work.

Response

Thanks for the opportunity, my group is very excited to see our discoveries being described in Wiki.

Reviewer 1 (Anonymous) comments


Review by anonymous peer reviewer ,
These assessment comments were submitted on , and refer to this previous version of the article

Conflicts of interest: none declared

This is a very well written article and the described information summarizes the mechanism and stepwise morphological changes during apoptotic cell disassembly. The authors may consider the minor change described below:

Apoptosis, a common type of programmed cell death, occurs in most tissues as part of homeostasis, development and in pathogenic processes such as inflammation and infection...
Response

The authors have made changes according to this suggestion.

Reviewer 2 comments


Review by Prof. dr. Christopher Gregory , Chair of Inflammatory Cell Biology / Director of the Centre for Inflammation Research, University of Edinburgh
These assessment comments were submitted on , and refer to this previous version of the article

Conflicts of interest: none declared

This is good overview of cell fragmentation during apoptosis by leading researchers in this field. It is both well-written and well-illustrated.
Response

We thank the reviewer for his comments.

I would suggest that the authors make specific reference to early descriptions of apoptotic bodies in vivo – the seminal work of Kerr, Wyllie and Currie (Br J Cancer, 1972) would make an excellent addition to the citations.
Response

We agree with the reviewer and we have now referenced Kerr, Wyllie and Currie (Br J Cancer 1972) accordingly in the revised manuscript.

The only other point I would make is that the authors imply – rightly – that apoptotic bodies are extracellular vesicles. However, the uninitiated reader could be left thinking from reading the current version of the manuscript that the smallest of such vesicles arising from apoptotic cells are around 1 μm in size, which is misleading, since smaller vesicles (often much smaller than 1 μm) are also produced by apoptotic cells. This is important since the term ‘apoptotic body’ has different meanings in the literature, ranging from a membrane delimited fragment produced by an apoptotic cell, to the residual apoptotic cell body. It would be helpful therefore if the authors provide their specific definition of an apoptotic body.
Response

The reviewer makes a very good point. We also agree the field needs to better define the term ‘apoptotic body’ consistently, and take into consideration that membrane-bound vesicles smaller than 1 micron are also released during apoptosis. Although not the best definition, for simplicity in this manuscript, we define apoptotic body as “membrane-bound apoptotic bodies (generally considered as approximately 1 to 5 microns in diameter”. To alert the reader that smaller vesicles can also arise from apoptotic cells, we have added “It should be noted that in addition to apoptotic bodies, membrane-bound vesicles that are smaller than 1 micron in diameter are also released during apoptosis” in the revised manuscript.

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