UTPA STEM/CBI Courses/Microbiology/Genetic Engineering Using Bacteria

Course Title: General Microbiology

Lecture Topic: Genetic Engineering in Bacteria

Instructor: Dr. Luis Materon

Institution: UTPA, Biology Department

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Challenge: There is currently a severe oil spill in the Gulf of Mexico that will affect wild life and business in the area. Task: Identify a gene that will degrade oil faster. The gene is to be inserted into a plasmid of an aquatic bacterial species such as Pelagibacter (The most abundant bacterial species in the ocean). The resulting recombinant bacteria having a new gene should accelerate the breakdown of oil on water and affected beaches. Adopt a protocol to isolate and insert a gene from Pseudomonas into Pelagibacter. Assess your contribution to reduce environmental damage from oil spill.

• Backwards Design

Course Objectives

• Primary Objectives- By the next class period students will be able to:
  • Understand
  • Discuss
  • Evaluate
  • Publish

• Sub Objectives- The objectives will require that students be able to:
  • Consult protocols
  • Experiment with enzymes
  • Observe growth
  • Discuss results
  • Write report

• Difficulties- Students may have difficulty:
  • Background knowledge in the molecular structure of DNA and other basic concepts
  • Getting access to lab after hours due to security regulations
  • Managing time
  • Reading results

• Real-World Contexts- There are many ways that students can use this material in the real-world, such as:
  • Research-oriented job.
  • Teaching

Model of Knowledge

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• Content Priorities
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  • Important to Do and Know
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  • Worth Being Familiar with
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Assessment of Learning

• Formative Assessment
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  • Homework (individual)
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• Summative Assessment
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Legacy Cycle

OBJECTIVE

By the next class period, students will be able to:

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The objectives will require that students be able to:

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THE CHALLENGE

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GENERATE IDEAS

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MULTIPLE PERSPECTIVES

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RESEARCH & REVISE

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TEST YOUR METTLE

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GO PUBLIC

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Test Your Mettle Quiz

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