Laboratory and Analytical Methods

1. Simple (glass) laboratory equipment and how to use it
2. Laboratory safety
3. w:Size exclusion chromatography
4. UV-VIS Spectrometry
5. IR spectrometry
6. Centrifugation and w:Ultracentrifugation
7. SDS PAGE technique: Proteins are treated with a material that gives them a charge in proportion to their mass. They are then placed at the starting line of a gel that has a current running through it. The molecules travel down the gel like electrons travel through an power line, this is called electrophoresis. Since the proteins have the same charge/mass ratio, they travel down the gel according to their size. The smallest proteins go the furthest. These results can then be compared in many different ways.
   • For more information see Details on Electrophoresis or Details on SDS-PAGE.
8. w:Chromatography
9. Gas chromatography and mass spectrometry
10. Gamma counting
11. Beta counting
12. w:Enzyme assays
13. w:PCR

• Metabolic pathways

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