English: Embryo Movements in different conditions. 0:00–0:11. NIC Embryo Movements at 24 hpf Noninjected control (NIC) zebrafish embryos at 24 hpf were filmed for one minute. Twitching was counted from individual embryos over multiple movies, as quantitated in Figure 1. 0:11–0:21. Effects of miR-153 Overexpression on Movement at 24 hpf Single cell zebrafish embryos were injected with miR-153 and filmed for one minute at 24 hpf. Twitching was counted from individual embryos over multiple movies, as quantitated in Figure 1. 0:22–0:32. Effects of Knockdown of miR-153 on Movement at 24 hpf Single cell zebrafish embryos were injected with miR-153MOs and filmed for one minute at 24 hpf. Twitching was counted from individual embryos over multiple movies, as quantitated in Figure 1. 0:33–0:42. Effects of Decreased SNAP-25 Expression on Movement at 24 hpf Single cell zebrafish embryos were injected with snap-25a,bMO and filmed for one minute at 24 hpf. Twitching was counted from individual embryos over multiple movies, as quantitated in Figure 1. 0:42–0:52. Effects of Increased SNAP-25 Expression on Movement at 24 hpf Single cell zebrafish embryos were injected with snap-25a,b mRNA and filmed for one minute at 24 hpf. Twitching was counted from individual embryos over multiple movies, as quantitated in Figure 1. 0:52–1:02. Effects of co-Injection of miR-153 and snap-25a,b on Movement at 24 hpf Single cell zebrafish embryos were co-injected with miR-153 and snap-25a,b mRNA and filmed for one minute at 24 hpf. Twitching was counted from individual embryos over multiple movies, as quantitated in Figure 1. 1:02–1:12. Effects of co-Injection of miR-153MO and snap-25a,bMO on Movement at 24 hpf Single cell zebrafish embryos were co-injected with miR-153MO and snap-25a,bMO and filmed for one minute at 24 hpf. Twitching was counted from individual embryos over multiple movies, as quantitated in Figure 1. 1:12–1:22. NIC Embryo Movements at 28 hpf Noninjected control (NIC) zebrafish embryos at 28 hpf were filmed for one minute at the same time that the following Movies were created. Twitching was counted from individual embryos, as quantitated in Figure 4C. 1:22–1:32. Effects of Botulinum Toxin Treatment on Movement at 28 hpf Single cell zebrafish embryos were injected with injection dye and treated with Botulinum toxin A at 27 hpf. After a 30 min treatment, embryos were washed and allowed to recuperate for 1 hour before being filmed. Twitching was counted from individual embryos, as quantitated in Figure 4C. 1:33–1:42. Effects of Botulinum Exposure and co-Injection of miR-153MO on Movement at 28 hpf Single cell zebrafish embryos were injected with miR-153MOs and treated with Botulinum toxin A at 27 hpf. After a 30 min treatment, embryos were washed and allowed to recuperate for 1 hour before being filmed. Twitching was counted from individual embryos, as quantitated in Figure 4C. 1:42–1:52. Effects of Botulinum Exposure and co-Injection of snap-25a,b mRNA on Movement at 28 hpf Single cell zebrafish embryos were injected with snap-25a,b mRNA and treated with Botulinum toxin A at 27 hpf. After a 30 min treatment, embryos were washed and allowed to recuperate for 1 hour before being filmed. Twitching was counted from individual embryos, as quantitated in Figure 4C.